Streptococcus mutans의 탄수화물 분해에 미치는 자일리톨의 효과
The effect of xylitol on the use of various carbohydrates by Streptococcus mutans
김지혜, 이영은, 정선영, 안상헌, 최연희, 송근배,
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김지혜 ( Kim Ji-Hye ) - 경북대학교 치의학전문대학원 예방치과학교실
이영은 ( Lee Young-Eun ) - 경북대학교 치의학전문대학원 예방치과학교실
정선영 ( Chung Sun-Young ) - 경북대학교 치의학전문대학원 예방치과학교실
안상헌 ( Ahn Sang-Hun ) - 동국대학교 의과대학 치과학교실
최연희 ( Choi Youn-Hee ) - 경북대학교 치의학전문대학원 예방치과학교실
송근배 ( Song Keun-Bae ) - 경북대학교 치의학전문대학원 예방치과학교실
KMID : 0355420100340010001
Abstract
Objective: Xylitol prevents caries by inhibiting the growth and virulence of Streptococcus mutans. However, most reports only examined the presence of specific carbohydrate such as glucose or sucrose. We therefore tested the effect of xylitol on S. mutans in several sugars.
Methods: Basal culture medium for S. mutans KCTC3065 was TYE medium containing 0.5% glucose (G-TYE). The strain was cultured in 10 ml basal culture medium adding 0.5% carbohydrates (sucrose, fructose, maltose) with or without 1% xylitol at 37℃ under 10% CO2 for 24 h. Cells were collected for analysis growth, acid production, synthesis of extracellular polysaccharides, and gene expression.
Results: Xylitol reduced the growth rate of S. mutans in the presence of G-TYE and maltose (p＜0.05), as well as the final growth of S. mutans in the presence of G-TYE, sucrose, and maltose (p＜0.05). Xylitol inhibited acid production in the presence of G-TYE and maltose (p＜0.05), but not in sucrose and fructose (p＞0.05). Xylitol reduced the synthesis of extracellular polysaccharides and the expression of gtfB and gtfD in all carbohydrates.
Conclusions: Xylitol inhibited growth and acid production of S. mutans in the presence of G-TYE and maltose, and decreased extracellular polysarccharides synthesis in all carbohydrates.
키워드
자일리톨;탄수화물
Streptococcus mutans;carbohydrates;xylitol
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